Superior MSRE-Free & Bisulfite-Free DNA Methylation Analysis close

For current pricing and to place an order contact us at info@ribomed.com.

Use of these products is described in the following publications:

  1. McCarthy et al. MethylMeter®: bisulfite-free quantitative and sensitive DNA methylation profiling and mutation detection in FFPE samples. Epigenomics 8(6), 747-765 (2016). http://www.futuremedicine.com/doi/pdf/10.2217/epi-2016-0004
  2. McCarthy D, Cotter P, Hanna M. MethylMeter: ah quantitative, sensitive, and bisulfite-free method for analysis of DNA methylation. In: DNA Methylation. Tatarinova T, Kerton O (Eds). InTech, Rijeka, Croatia, 93–116 (2012). https://www.researchgate.net/publication/221929219_MethylMeterr_A_Quantitative_Sensitive_and_Bisulfite-Free_Method_for_Analysis_of_DNA_Methylation

MethylMagnet®  Kits

Kits with magnetic bead bound MethylMagnet protein for separating methylated and unmethylated DNA.

Catalog Number Quantity
MM101-K30 MethylMagnet® Kit: 30 samples 30

CAPS Kits – Format II (Fluorescent Detection)

Kits for Detecting DNA targets with Coupled Abscription PCR Signaling (CAPS) – NO TARGET PRIMERS

CAPS – K60 CAPS Kit Format II:  60 reactions 60
CAPS – K150 CAPS Kit Format II:  150 reactions  150

Target-Specific MethylMeter® and CAPS ™ Kits – Format II (Fluorescent Detection)


Description: These are target or biomarker panel specific kits already containing target-specific CAPS primers. MethylMeter is used to measure DNA methylation and includes MethylMagnet and CAPS. Each MethylMeter kit contains reagent for analysis of 12 samples and calibrators, and CAPS reactions on both the methylated and unmethylated fractions for each of the genes/CpG islands included in the assay.  Mutation detection only requires CAPS.

Catalog Number

Description

Quantity

 

MM-MGMT-I MethylMeter-MGMT-I 12
MM-MGMT-II MethylMeter-MGMT-II 12
MM-GCIMP MethylMeter-GCIMP 12
MM-GliomaSTRAT MethylMeter-GliomaSTRAT 12
CK-IDH1 R132H CAPS-IDH1 WT and R132H detection kit 12
MM-MGMT-HFE MethylMeter-HFE 12
MM-hMLH1 MethylMeter-hMLH1 12
MM-MAL MethylMeter-MAL 12
MM-SOWAHA MethylMeter-SOWAHA 12
MM-SNRPN MethylMeter-SNRPN 12
MM-DAPK1 MethylMeter-DAPK1 12
MM-RBI MethylMeter-RBI 12

Target Specific CAPS primer sets

Catalog Number

Description

Quantity

CP-UP Universal Primer Set 60
CP-MGMT-II MGMT-II Upstream CAPS Primers 60
CP-MGMT-I MGMT-I Downstream CAPS Primers 60
CP-MAL MAL CAPS Primers 60
CP-SOWAHA SOWAHA CAPS Primers 60
CP-IDH1-WT IDH-1 WT  CAPS Primers 60
CP-IDH1-R132H IDH1-R132H CAPS Primers 60
CP-HFE HFE  CAPS Primers 60
CP-HFE-AV HFE Alul Validation CAPS Primers 60
CP-SNRPN  SNRPN CAPS Primers 60
CP-RB1-I RB1 imprinted CAPS Primers 60
CP-DAPK1  DAPK1 CAPS Primers 60
CP-MLH1  MLH1 CAPS Primers 60
CP-OR10K1  OR10K1 CAPS Primers 60
CP-VTRNA2-1  VTRNA2-1 CAPS Primers 60
CP-CDH1  CDH1 CAPS Primers 60
CP-KCTD5  KCTD5 CAPS Primers 60

MethylMeter®  Kit Individual Components

Catalog Number

Description

Quantity

 

MM101 MethylMagnet-GST-MBD Protein 100 μg
FP-002 Abscriptase II 60
FP-003 CAPS DNA Polymerase 60
MB-001 Molecular Beacon 1 160
MM301-HD HeLa DNA 15 μg
MM302-MHD Methylated HeLA DNA 15 μg

Abscription/Transcription Initiators: RNA dinucleotides

Catalog Number

Description

Quantity

I11-002 Adenylyl(3’—5′)adenosine, ApA 2 umole
I14-002 Adenylyl(3’—5′)uridine, ApU 2
IP14-002  5′-phospho-Adenylyl(3’—5′)uridine, pApU 2
I21-002  Cytidylyl (3’—5′)adenosine, CpA 2
I41-002 Uridylyl (3’—5′)adenosine, UpA 2
I42-002 Uridylyl (3’—5′)cytidine, UpC 2
I43-002 Uridylyl (3’—5′)guanosine, UpG 2

Abscription/Transcription Terminators (3′-OMe-NTPs)

Catalog Number

Description

Quantity

TM01-005  3′-O-Methyl Adenosine-5′-Triphosphate, 3′-OMeATP 5 umole
TM02-005  3′-O-Methyl Cytidine-5′-Triphosphate, 3′-OMeCTP 5
TM03-005 3′-O-Methyl Guanosine-5′-Triphosphate, 3′-OMeGTP 5
TM04-005  3′-O-Methyl Uridine-5′-Triphosphate, 3′-OMeUTP 5
TM01D-005  3′-O-Methyl-8-(2,4 Dinitrophenyl-mercapto) Adenosine-5′-Triphosphate, 3′-OMe-DNP-S-ATP

Equipment

Catalog Number

Description

Quantity

MR24-01 24-Tube Magnetic Rack 1
MTC6-01 Six-Plate TLC Chamber 1

 

Services

Testing Services 

Description

In-house Testing & Custom Assays

Methylated CpG islands using native DNA.
Bisulfite treatment not required.
Developed for multiple islands, or high throughput screening.
Can be done for single islands on multiple samples.
Customers only supply purified DNA & information on target of interest.
Custom Kits

Description

Custom Kit Development

Custom development of testing kits for use by the customer in their own laboratories.
Customer need only provide information on the targets of interest.
 Contact us at info@ribomed.com